Measurement of ultrasound-enhanced diffusion coefficient of nanoparticles in an agarose hydrogel

An experimental study has been performed to measure the effect of ultrasound on nanoparticle diffusion in an agarose hydrogel. Agarose hydrogel is often used as a simulant for biofilms and certain biological tissues, such as muscle and brain tissue. The work was motivated by recent experiments indicating that ultrasonic excitation of moderate intensity can significantly enhance nanoparticle diffusion in a hydrogel. The objective of the current study was to obtain detailed measurements of the effect of ultrasound on nanoparticle diffusion in comparison to the molecular diffusion in the absence of acoustic excitation. Experiments were conducted with 1 MHz ultrasound waves and nanoparticle diameters of 20 and 100 nm, using fluorescent imaging to measure particle concentration distribution. Under ultrasound exposure, the experiments yield estimates for both acoustic diffusion coefficients as well as acoustic streaming velocity within the hydrogel. Measured values of acoustic streaming velocity were on the order of 0.1 μm/s, which agree well with a theoretical estimate. Measured values of the acoustic diffusion coefficient were found to be 74% larger than the molecular diffusion coefficient of the nanoparticles for 20 nm particles and 133% larger than the molecular diffusion coefficient for 100 nm particles.