Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant DNA techniques in medicine.

G K Brown1

  • 1Department of Paediatrics, University of Melbourne, Victoria, Australia.

Australian Paediatric Journal
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for induction of humoral and cytotoxic immune responses against devil facial tumor disease cells in Tasmanian devils (Sarcophilus harrisii) immunized with killed cell preparations.

Vaccine·2015
Same author

High phylogenetic diversity of the cat flea (Ctenocephalides felis) at two mitochondrial DNA markers.

Medical and veterinary entomology·2014
Same author

'Cysticercosis storm' in feedlot cattle in north-west New South Wales.

Australian veterinary journal·2013
Same author

Changes in problem-solving appraisal after cognitive therapy for the prevention of suicide.

Psychological medicine·2011
Same author

Reversible infantile respiratory chain deficiency is a unique, genetically heterogenous mitochondrial disease.

Journal of medical genetics·2011
Same author

Two cases with progressive cystic leukoencephalopathy.

Neuropediatrics·2009

Recombinant DNA technology revolutionizes genetic disease diagnosis by enabling direct detection of mutations or indirect linkage analysis. This powerful tool also facilitates

Area of Science:

  • Biotechnology
  • Medical Genetics
  • Molecular Biology

Background:

  • Recombinant DNA technology has significantly advanced medical diagnostics.
  • Genetic diseases pose a considerable diagnostic challenge.

Purpose of the Study:

  • To review the medical applications of recombinant DNA technology.
  • To highlight its impact on diagnosing and understanding genetic diseases.

Main Methods:

  • Direct gene defect detection using DNA probes when the gene is known.
  • Indirect diagnosis via linkage analysis with restriction fragment length polymorphisms (RFLPs) for unknown genes.
  • Application of 'reverse' genetics to identify and analyze disease-causing genes.

Main Results:

Related Experiment Videos

  • Recombinant DNA techniques enable direct diagnosis of genetic diseases when gene defects are known.
  • Linkage analysis provides diagnostic capabilities even when genes are unknown or mutations are difficult to detect.
  • Reverse genetics allows for the identification and characterization of disease-related genes irrespective of prior functional knowledge.

Conclusions:

  • Recombinant DNA technology offers versatile approaches for diagnosing genetic disorders.
  • It is instrumental in unraveling the genetic basis of diseases through both direct and indirect methods.
  • The technology facilitates a deeper understanding of gene function and its role in disease pathogenesis.