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The Functional Mechanisms of miR-30b-5p in Acute Lung Injury in Children

Ting Zhou1, Yong-Li Chen2

  • 1Department of Pediatrics, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|January 3, 2019
PubMed

Insights

MicroRNA (miRNA) miR-30b-5p levels decrease in pediatric acute lung injury (ALI). Lower miR-30b-5p correlates with increased inflammation markers and suggests it may be a therapeutic target for ALI.

Area of Science:

  • Pediatric critical care medicine
  • Molecular biology
  • Pulmonology

Background:

  • Acute lung injury (ALI) in children involves complex inflammation.
  • MicroRNAs (miRNAs) are implicated in ALI pathogenesis.
  • The specific role of miR-30b-5p in ALI remains unclear.

Purpose of the Study:

  • To investigate miR-30b-5p and suppressor of cytokine signaling 3 (SOCS3) alterations in pediatric ALI.
  • To examine these factors in a lipopolysaccharide (LPS)-induced mouse model of ALI.
  • To explore the relationship between miR-30b-5p and SOCS3.

Main Methods:

  • Quantified miR-30b-5p, SOCS3, fractalkine (FKN), TNF-α, NF-κB, IL-6, and IL-8 using ELISA, western blot, and qRT-PCR.
  • Assessed alveolar permeability and lung tissue W/D ratio.
  • Evaluated inflammation and apoptosis via H&E and TUNEL assays.
  • Confirmed SOCS3 as a miR-30a-5p target using dual-luciferase reporter assays.

Main Results:

  • Decreased miR-30b-5p and increased SOCS3, FKN, TNF-α, NF-κB, IL-6, and IL-8 were observed in ALI patients and the mouse model.
  • LPS challenge increased alveolar permeability, lung W/D ratio, and activated the NF-κB pathway.
  • SOCS3 was validated as a direct target of miR-30a-5p.

Conclusions:

  • miR-30b-5p plays a significant role in ALI-associated inflammation.
  • miR-30b-5p may represent a potential therapeutic target for pediatric ALI.
  • The miR-30b-5p/SOCS3 axis is involved in ALI pathogenesis.

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