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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

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Published on: November 26, 2017

Fluorogenic Detection and Characterization of Proteins by Aggregation-Induced Emission Methods.

Sheng Xie¹, Alex Y H Wong¹, Sijie Chen¹

¹Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, S.A.R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|January 3, 2019

Summary

Aggregation-induced emission (AIE) fluorescence offers novel, sensitive methods for protein detection and characterization. This review highlights AIE strategies for analyzing protein behavior and interactions, advancing protein science.

Keywords:

aggregation dyes/pigments fluorescence noncovalent interactions proteins

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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

Area of Science:

Biochemistry and Molecular Biology
Biophysical Chemistry
Analytical Chemistry

Background:

Proteins are crucial biomacromolecules essential for biological functions and disease diagnostics.
Traditional fluorescence techniques are widely used for protein studies but have limitations.
Aggregation-induced emission (AIE) dyes offer a unique 'turn-on' fluorescence mechanism based on restricted intramolecular motion.

Purpose of the Study:

To review the applications of aggregation-induced emission (AIE) fluorescence in protein science.
To highlight novel fluorogenic strategies enabled by AIE for sensitive and selective protein analysis.
To discuss AIE applications in protein detection, localization, quantification, conformational studies, ligand interactions, and enzyme activity evaluation.

Main Methods:

Review of existing literature on AIE fluorescence applications in protein science.
Focus on AIE strategies utilizing the 'turn-on' mechanism triggered by restricted intramolecular motion.
Categorization of applications including protein detection, localization, quantification, conformational transitions, protein-ligand interactions, and enzyme activity.

Main Results:

AIE fluorescence provides sensitive, selective, and reliable methods for protein analysis.
AIE strategies enable novel approaches for probing protein conformational changes and interactions.
AIE is effective for evaluating enzyme activities and characterizing protein-ligand binding.

Conclusions:

AIE fluorescence represents a powerful and emerging tool for comprehensive protein characterization.
The unique properties of AIE dyes facilitate advanced fluorogenic strategies in protein science.
Further research into AIE applications holds significant promise for diagnostics and fundamental biological understanding.