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Updated: Jan 31, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
Published on: September 6, 2013
Super-resolution microscopy demystified
Lothar Schermelleh1, Alexia Ferrand2, Thomas Huser3
1Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, Oxford, UK. lothar.schermelleh@bioch.ox.ac.uk.
Abstract:
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
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