Histological and micro-computed tomography evaluations of newly formed bone after maxillary sinus augmentation using a xenograft with similar density and mineral content of bone: An experimental study in rabbits

The objective of this study was to evaluate possible differences in the assessment of bone formation between histological and micro-computed tomography (CT) analyses in maxillary sinuses augmented with a xenograft with similar density and mineral content of bone. A collagen membrane was placed subjacent the elevated sinus mucosa at the test sites of 18 rabbits, and the elevated spaces were filled with xenograft. The antrostomy was covered with collagen membranes, bilaterally. Six rabbits per group were sacrificed after 2, 4, and 8 weeks of healing. Biopsies were retrieved and scanned in a high-resolution micro-CT at two different gray thresholds. Histological assessments were subsequently performed. At the histological analyses, bone increased over time, from 7.5 ± 2.4% to 27.0 ± 5.3%, between 2 and 8 weeks of healing. The highest content of bone was found close to the sinus bone walls, whereas the middle regions contained lower amounts. At the micro-CT analyses, discrepancies were found in bone content percentages compared with the histological analyses, especially after 2 weeks of healing and within the middle regions of the sinus, in which new bone was ~15-22% at the micro-CT analyses and only 1.6% at the histological evaluation. The outcomes of a micro-CT analysis performed in an early phase of healing may be altered when a resorbable bone substitute with similar density and mineral content of bone is applied.