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The irreversibility of thiamin transport in Saccharomyces cerevisiae
T Ruml1, L Silhánková, P Rauch
1Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague.
Folia Microbiologica
|January 1, 1988
Abstract:
Neither exit nor counterflow efflux of thiamin, taken up previously by an active transport, were found in Saccharomyces cerevisiae, in either the wild type or a mutant with a lower rate of thiamin phosphorylation. Complete inhibition of thiamin phosphorylation by oxythiamin did not lead to any release of thiamin taken up by the cell.