Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Isothermal Processes01:21

Isothermal Processes

5.0K
A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
5.0K
Drug Absorption Mechanism: Carrier-Mediated Membrane Transport01:19

Drug Absorption Mechanism: Carrier-Mediated Membrane Transport

6.1K
Certain large, lipid-insoluble drug molecules that resemble amino acids, peptides, or glucose, require specialized carrier proteins to facilitate their diffusion across cell membranes. This transport can occur through either facilitated diffusion, which does not require energy input, or active transport, which does require energy input.
Facilitated diffusion is a passive process that utilizes human Solute Carrier (SLC) transporters. These transporters bind to the drug, undergo structural...
6.1K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

110.9K
Overview
110.9K
Feedback Loops01:01

Feedback Loops

64.4K
In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
64.4K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.3K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.3K
Open and closed-loop control systems01:17

Open and closed-loop control systems

1.7K
Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
An open-loop control system operates without feedback from the output. It consists of two primary elements: the controller and the controlled process. The controller receives an input signal...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Nanoconfined, Hydrogel-Partitioned LAMP Platform for Rapid, Extraction-Free Quantification of <i>Mycoplasma pneumoniae</i>.

ACS infectious diseases·2026
Same author

A Droplet-Free and Oil-Free Microfluidics Based on Self-Pumping Hydrogel for Point-of-Care Multiplex Digital Nucleic Acid Detection.

Analytical chemistry·2026
Same author

Revolutionizing Fresh Food Quality Control in Supply Chains With Machine Learning: Current Advances and Future Challenges.

Comprehensive reviews in food science and food safety·2025
Same author

Compartmentalization and Aggregation of Biomolecular Condensates in Crowded Hydrogels for Enhanced Nucleic Acid Diagnosis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Hygroscopic-Evaporative Generator for Multiform Energy Harvesting from Environment and Food Storage.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Hydrogel-based electrochemical LAMP sensor for rapid, contamination-free and ultrasensitive point-of-care detection of <i>Vibrio parahaemolyticus</i>.

Chemical communications (Cambridge, England)·2025

Related Experiment Video

Updated: Jan 31, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane.

Xingyu Lin1, Xiao Huang1, Katharina Urmann1

  • 1Linde + Robinson Laboratories , California Institute of Technology , Pasadena , California 91125 , United States.

ACS Sensors
|January 4, 2019
PubMed
Summary

This study introduces digital loop-mediated isothermal amplification (LAMP) on a low-cost membrane, enabling precise DNA quantification without specialized equipment. This method offers a simplified, affordable approach for various bioassays and point-of-care applications.

Keywords:
PCRdigital LAMPdropletsmembranemicrofluidicnucleic acidpaper-based analytical device

More Related Videos

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

17.2K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.9K

Related Experiment Videos

Last Updated: Jan 31, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K
A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

17.2K
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.9K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • Digital loop-mediated isothermal amplification (LAMP) offers precise nucleic acid quantification but often requires complex instrumentation.
  • Existing digital LAMP methods can be costly and inaccessible for widespread use, limiting their application in resource-limited settings.

Purpose of the Study:

  • To develop an inexpensive and simplified digital LAMP platform using commercial membranes.
  • To enable absolute quantification of DNA and RNA targets without specialized equipment.
  • To demonstrate the potential for point-of-care applications and bioassays.

Main Methods:

  • Utilized commercial track-etched polycarbonate (PCTE) membranes for digital LAMP and reverse-transcription LAMP (RT-LAMP).
  • Developed a facile peel-off process for droplet formation, enabling each pore to act as a nanoreactor.
  • Analyzed theoretical error using Random Distribution Model and Multivolume Theory based on pore size distribution.

Main Results:

  • Achieved absolute quantification of bacterial genomic DNA over a dynamic range of 11 to 1.1 × 10^5 copies/μL.
  • Successfully performed one-step digital RT-LAMP for MS2 virus quantification in wastewater.
  • Demonstrated clear distinction between positive and negative pores with 100-fold fluorescence difference using novel probes.

Conclusions:

  • The developed membrane-based digital LAMP system is a cost-effective (<$0.10/piece) and simplified method for nucleic acid quantification.
  • This platform facilitates digital quantification, single-cell analysis, and other bioassays for point-of-care users and common laboratories.
  • The system offers a flexible and accessible alternative to traditional digital PCR or chip-based methods.