Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Absolute Value Inequalities01:23

Absolute Value Inequalities

333
The absolute value is a mathematical tool that represents the distance of a number from zero on the number line, regardless of its sign. In the context of inequalities, absolute value expressions help define a range of permissible values or boundaries for a variable. These inequalities are commonly used in scientific modeling and data interpretation, where variability within or beyond a certain threshold must be captured precisely.An absolute value inequality of the form ∣x∣ ≤...
333
Mean Absolute Deviation01:13

Mean Absolute Deviation

3.3K
The mean absolute deviation is also a measure of the variability of data in a sample. It is the absolute value of the average difference between the data values and the mean.
Let us consider a dataset containing the number of unsold cupcakes in five shops: 10, 15, 8, 7, and 10. Initially, calculate the sample mean. Then calculate the deviation, or the difference, between each data value and the mean. Next, the absolute values of these deviations are added and divided by the sample size to...
3.3K
Proteomics01:33

Proteomics

9.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.7K
Absolute and Local Extreme Values01:22

Absolute and Local Extreme Values

73
The highest and lowest values of a function, relative to a reference axis, are known as extreme values. These include absolute maximum and absolute minimum values, which represent the highest and lowest points the function reaches across its entire domain. Within a restricted portion of the function, the highest and lowest values are referred to as local maximum and local minimum values, respectively.Periodic functions, such as sine and cosine, show extreme values at infinitely many points due...
73
Absolute Motion Analysis- General Plane Motion01:24

Absolute Motion Analysis- General Plane Motion

563
Visualize a drone, with its propellers spinning rapidly, hovering mid-air. The fascinating movements and operations of this drone can be comprehended by applying the principle of general plane motion.
As the drone's propellers rotate, an upward force is generated that counteracts the force of gravity, enabling the drone to lift off from the ground. This initial movement of the drone is along a straight path, representing a form of translational motion. In this phase, every point on the...
563
Bioavailability Study Design: Absolute Versus Relative Bioavailability01:27

Bioavailability Study Design: Absolute Versus Relative Bioavailability

339
Bioavailability is a crucial pharmacokinetic parameter that quantifies the proportion of an administered drug that reaches the systemic circulation and is available for therapeutic action. Regulatory agencies mandate the assessment of bioavailability, typically measured as the area under the drug plasma concentration-versus-time curve (AUC), to ensure the efficacy and safety of pharmaceutical products. These evaluations are categorized as absolute and relative bioavailability studies.Absolute...
339

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuropeptide Diversity Encoded in Newly Sequenced Crustacean Genomes Reveals Signaling Roles during Feeding.

ACS chemical neuroscience·2026
Same author

Mass Spectrometry-Based Peptidomics for the Discovery and Profiling of Endogenous Peptides in Crustacean Hemolymph.

ACS omega·2026
Same author

Spatially resolved lipid compartmentalization in human pancreatic islets revealed by high-resolution ultra-low-flow-rate desorption electrospray ionization mass spectrometry imaging.

Analytical and bioanalytical chemistry·2026
Same author

Decoding stereospecific metabolic regulation of protein modifications.

Nature chemistry·2026
Same author

Bridging genomes and peptidomes: hybrid sequencing reveals conserved bioactive peptides in crustaceans.

bioRxiv : the preprint server for biology·2026
Same author

Molecular insights into DDX3X-androgen receptor mRNA regulation via non-canonical G-quadruplex in castration-resistant prostate cancer.

Oncogene·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.6K

HOTMAQ: A Multiplexed Absolute Quantification Method for Targeted Proteomics.

Xiaofang Zhong1, Qinying Yu1, Fengfei Ma1

  • 1School of Pharmacy , University of Wisconsin-Madison , Madison , Wisconsin 53705 , United States.

Analytical Chemistry
|January 5, 2019
PubMed
Summary
This summary is machine-generated.

We developed a new Hybrid Offset-Triggered Multiplex Absolute Quantification (HOTMAQ) strategy for precise protein measurement. This method significantly enhances throughput for targeted proteomics, aiding biomarker discovery in diseases like Alzheimer's.

More Related Videos

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

12.0K
A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

8.4K

Related Experiment Videos

Last Updated: Jan 31, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.6K
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

12.0K
A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

8.4K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Targeted proteomics faces challenges in absolute quantification, including low specificity, limited throughput, and wide dynamic ranges.
  • Accurate protein quantification is crucial for biomarker discovery and disease research.

Purpose of the Study:

  • To develop a novel strategy for high-throughput and accurate absolute quantification in targeted proteomics.
  • To address the limitations of existing methods in specificity, throughput, and dynamic range.

Main Methods:

  • Developed a Hybrid Offset-Triggered Multiplex Absolute Quantification (HOTMAQ) strategy.
  • Combined mass difference and isobaric tags for internal standard curve construction.
  • Utilized MS1 precursor scan, MS2 identification, and SPS-MS3 for accurate quantification.

Main Results:

  • Achieved up to a 12-fold increase in analytical throughput for targeted quantitative proteomics.
  • Demonstrated high accuracy in quantifying target proteins using the HOTMAQ strategy.
  • Successfully verified candidate protein biomarkers in preclinical Alzheimer's disease models.

Conclusions:

  • The HOTMAQ strategy offers enhanced throughput and quantitative performance for targeted proteomics.
  • This approach is broadly applicable to targeted peptidomics, proteomics, and phosphoproteomics.
  • HOTMAQ provides a flexible and accurate solution for complex biological samples.