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A Review on Quantitative Multiplexed Proteomics.

Nishant Pappireddi1,2, Lance Martin1,2, Martin Wühr1,2

  • 1Department of Molecular Biology, Princeton University, Icahn Laboratory, #246, Washington Road, Princeton, NJ, 08544, USA.

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Summary
This summary is machine-generated.

Multiplexed proteomics using isobaric tags allows for the quantification of thousands of proteins across multiple samples. Future directions include combining multiplexing with targeted and data-independent acquisition for enhanced protein quantification.

Keywords:
analytical methodsisotopic labelingmass spectrometryproteinsproteomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Mass spectrometry-based proteomics has advanced significantly, enabling large-scale protein detection and quantification.
  • Isobaric tags revolutionized global protein quantification by allowing simultaneous measurement across multiple samples, a technique termed multiplexed proteomics.

Purpose of the Study:

  • To discuss and compare various multiplexed proteomics techniques, including their principles, advantages, and disadvantages.
  • To explore the potential of combining multiplexing with targeted proteomics for quantifying low-abundance proteins.
  • To investigate the fusion of multiplexed proteomics with data-independent acquisition for high-throughput sample comparison.

Main Methods:

  • Review and comparison of existing multiplexed proteomics techniques based on isobaric tags.
  • Discussion of emerging strategies combining multiplexing with targeted and data-independent acquisition methods.

Main Results:

  • Isobaric tag-based multiplexed proteomics offers robust global protein quantification across samples.
  • Combining multiplexing with targeted approaches may improve quantification of low-abundance proteins.
  • Fusing multiplexing with data-independent acquisition could enable large-scale sample comparisons with high precision and accuracy.

Conclusions:

  • Multiplexed proteomics is a powerful tool for large-scale protein quantification.
  • Emerging combinations of multiplexing with other advanced MS techniques promise enhanced capabilities for protein analysis.
  • Future proteomics studies can benefit from these integrated approaches for comprehensive biological insights.