Related Experiment Video
Updated: Jan 31, 2026
10:28
Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
22.2K
SubCellBarCode: Proteome-wide Mapping of Protein Localization and Relocalization
Lukas Minus Orre1, Mattias Vesterlund1, Yanbo Pan1
1Department of Oncology and Pathology, Karolinska Institutet, Science for Life Laboratory, 17165 Solna, Sweden.
Molecular Cell
|January 5, 2019
Summary
This study maps the subcellular locations of thousands of proteins across cell types using mass spectrometry. Most proteins reside in one location, rarely altered by splicing, with cell identity linked to surface proteins.
Area of Science:
- Proteomics
- Cell Biology
- Bioinformatics
Background:
- Subcellular localization dictates protein function, but a comprehensive proteome-wide view is lacking.
- Understanding spatial proteome organization is crucial for cell biology.
Purpose of the Study:
- To create a proteome-wide map of subcellular localization across multiple cell lines.
- To investigate factors influencing protein localization, including alternative splicing, protein domains, and cell type.
- To analyze protein relocalization dynamics in response to external stimuli.
Main Methods:
- Development of a mass spectrometry-based analysis pipeline.
- Classification of over 83,000 protein localizations across five cell lines.
- Correlation profiling to analyze co-localization and identify localization determinants.
Main Results:
- The majority of proteins exhibit a single primary subcellular location.
- Alternative splicing has a minimal impact on subcellular localization.
- Cell-type-specific localization is most pronounced for proteins interacting with the extracellular environment.
- Protein relocalization was observed upon growth factor inhibition.
Conclusions:
- The study provides a comprehensive resource for subcellular proteome organization.
- Spatial proteome organization is complex, with most proteins maintaining a stable location.
- Cellular identity is significantly influenced by cell surface protein expression patterns.
Related Concept Videos
01:42Microtubule Associated Proteins (MAPs)
5.9K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
5.9K
01:33Proteomics
9.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.7K
01:11Genome-wide Association Studies-GWAS
15.7K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
15.7K
01:22Local Attraction
379
Local attraction refers to disturbances in compass readings caused by magnetic influences from nearby objects such as metal fences, buried pipes, vehicles, buildings, power lines, or natural iron ore deposits. Small items like wristwatches, steel tools, or belt buckles can also interfere with the compass by creating local magnetic fields that distort the Earth's natural magnetic field. These distortions lead to inaccurate readings, posing navigation and land surveying challenges.Local...
379
02:26Protein Networks
4.5K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.5K
01:29Plotting of Topographic Maps
519
Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...
519