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CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation
Aimee Malzahn1, Yong Zhang2, Yiping Qi3,4
1Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, MD, USA.
Abstract:
CRISPR systems have greatly promoted research in genome editing and transcriptional regulation. CRISPR-based transcriptional repression and activation systems will be valuable for applications in engineering plant immunity, boosting metabolic production, and enhancing our knowledge of gene regulatory networks. Multiplexing of CRISPR allows multiple genes to be targeted without significant additional effort. Here, we describe our CRISPR-Act2.0 system which is an improved multiplexing transcriptional activation system in plants.
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