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Updated: Jan 31, 2026

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Published on: July 27, 2013
Three-Color Simultaneous Live Imaging of Autophagy-Related Structures
Hiroyuki Ueda1,2, Ouin Kunitaki1, Maho Hamasaki3
1Department of Genetics, Osaka University Graduate School of Medicine, Osaka, Japan.
Abstract:
Simultaneous live cell imaging of multiple proteins helps to analyze mobility and interactions among proteins over time. Since autophagosomes depend on other organelles for their formation, it is necessary to observe this process with multiple fluorsphores to mark multiple organelles and the autophagosomes. To do so, we set up three cameras on one microscope to be able to acquire three colors at the same time. Here we describe the setup using a Yokogawa spinning disk confocal microscope (CSU-W1) with Andor TuCam system attaching 3 × Zyla 4.2 CMOS cameras (Andor) and detail the method for acquiring live images.
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