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Updated: Jan 31, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
Published on: November 30, 2022
Imaging the Dynamics of Mitophagy in Live Cells
Andrew S Moore1, Erika L F Holzbaur2
1Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Abstract:
Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays to quantitatively assess mitophagy dynamics in real time. This protocol describes both chemical and optogenetic techniques to induce mitochondrial damage with high spatial and temporal control. Using these assays, mitochondria can be tracked from before they sustain damage up to their engulfment by autophagosomes and acidification by lysosomes.
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