Optimized fabrication of newly cholesterol biosensor based on nanocellulose

Area of Science:

• Electrochemistry
• Biosensor Development
• Nanomaterials Science

Background:

• Cholesterol monitoring is crucial for cardiovascular health.
• Existing biosensors often face limitations in sensitivity and stability.
• Development of novel electrode materials is key to improving biosensor performance.

Purpose of the Study:

• To develop a novel and sensitive electrochemical cholesterol biosensor.
• To optimize biosensor performance using response surface methodology.
• To evaluate the biosensor's sensitivity, linear range, LOD, reproducibility, and selectivity.

Main Methods:

• Immobilization of cholesterol oxidase (ChOx) on a polyaniline/crystalline nanocellulose/ionic liquid modified Screen-Printed Electrode (PANi/CNC/IL/SPE).
• Preparation of crystalline nanocellulose from Semantan bamboo via acid hydrolysis.
• Synthesis of PANi/CNC nanocomposite via in situ oxidative polymerization.
• Optimization of biosensor parameters using Response Surface Methodology (RSM).
• Electrochemical characterization and performance evaluation.

Main Results:

• The developed PANi/CNC/IL/GLU/ChOx-modified electrode exhibited high sensitivity (35.19 μA mM⁻¹ cm⁻²).
• A wide dynamic linear range (1 μM to 12 mM) with a low Limit of Detection (LOD) of 0.48 μM was achieved.
• The biosensor demonstrated acceptable reproducibility (RSDs ≤3.76%) and repeatability (RSDs ≤3.31%) with minimal interference.

Conclusions:

• The novel PANi/CNC/IL/SPE platform provides a highly sensitive and stable matrix for cholesterol biosensing.
• The optimized biosensor shows significant potential for accurate and reliable cholesterol determination in various applications.
• This work highlights the synergistic benefits of combining polyaniline, nanocellulose, and ionic liquid for advanced electrochemical biosensor design.