Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Endocrine Signaling01:45

Endocrine Signaling

68.1K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
68.1K
Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Synaptic Signaling01:12

Synaptic Signaling

79.5K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.5K
Paracrine Signaling01:21

Paracrine Signaling

59.6K
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
59.6K
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

53.5K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
53.5K
Bacterial Signaling01:30

Bacterial Signaling

40.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Pick fold in tau filaments from human MAPT mutants.

Acta neuropathologica·2026
Same author

HKDC1 contributes to aberrant lysosome-mitochondria contact in Niemann-Pick disease type C.

bioRxiv : the preprint server for biology·2026
Same author

A macroevolution-inspired approach to reveal novel antibiotic resistance mechanisms.

eLife·2026
Same author

Macrophages self-generate and refine chemotactic gradients during migration towards complement C5a.

PLoS biology·2026
Same author

Integrase anchors viral RNA to the HIV-1 capsid interior.

Nature·2026
Same author

LAMP1 and LAMP2A localise to axonal organelles with distinct motility dynamics and partially overlapping molecular signatures in human neurons.

Journal of cell science·2026

Related Experiment Video

Updated: Jan 31, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

8.3K

The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module.

John M E Nichols1, Peggy Paschke2, Sew Peak-Chew3

  • 1Cell Biology Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK; MRC Laboratory for Molecular Cell Biology, University College London, Gower St., London WC1E 6BT, UK.

Developmental Cell
|January 8, 2019
PubMed
Summary

Chemoattractant signaling activates cell movement. Atypical MAP kinase ErkB phosphorylates key proteins, regulating cell migration and orientation in Dictyostelium discoideum, revealing its central role in chemotaxis.

Keywords:
Dictyostelium discoideumMAPK signalingchemotaxisphosphoproteomicsprotein kinaseprotein phosphorylationsignal transduction

More Related Videos

Recording Human Electrocorticographic ECoG Signals for Neuroscientific Research and Real-time Functional Cortical Mapping
13:32

Recording Human Electrocorticographic ECoG Signals for Neuroscientific Research and Real-time Functional Cortical Mapping

Published on: June 26, 2012

26.8K
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells
11:00

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Published on: July 6, 2019

7.2K

Related Experiment Videos

Last Updated: Jan 31, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

8.3K
Recording Human Electrocorticographic ECoG Signals for Neuroscientific Research and Real-time Functional Cortical Mapping
13:32

Recording Human Electrocorticographic ECoG Signals for Neuroscientific Research and Real-time Functional Cortical Mapping

Published on: June 26, 2012

26.8K
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells
11:00

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Published on: July 6, 2019

7.2K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Chemotaxis, or directed cell migration, is crucial for development and immunity.
  • Chemoattractant receptor signaling initiates cytoskeletal rearrangements for cell movement.
  • The precise molecular mechanisms integrating signaling pathways remain incompletely understood.

Purpose of the Study:

  • To investigate the role of atypical MAP kinase ErkB in chemotaxis.
  • To identify ErkB substrates and their function in Dictyostelium cell signaling.
  • To elucidate the integration of ErkB signaling with G-protein-coupled receptor pathways.

Main Methods:

  • Phosphoproteomic analysis of Dictyostelium cells stimulated with chemoattractants.
  • Site-directed mutagenesis to study ErkB phosphorylation motifs.
  • Analysis of ErkB null mutant cells for migration defects.

Main Results:

  • Chemoattractants induce phosphorylation of ~80 proteins, primarily at an [S/T]PR motif by ErkB.
  • ErkB-mediated phosphorylations are persistent and do not adapt to sustained chemoattractant stimulation.
  • ErkB targets are predominantly signaling proteins, not direct cytoskeletal regulators, indicating a 'regulator of regulators' role.

Conclusions:

  • ErkB plays a central role in directing chemotaxis by phosphorylating a conserved set of signaling proteins.
  • ErkB integrates dynamic autophosphorylation with G-protein-coupled receptor signaling.
  • ErkB null cells exhibit impaired migration and orientation, underscoring its importance in chemotaxis.