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Updated: Jan 31, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
Insulin-like growth factor-II mRNA-binding protein 3 immunocytochemical expression in direct endometrial brushings:
Yoshiaki Norimatsu1, Kenji Yanoh2, Yoshinobu Maeda3
1Departments of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Tobe-cho, Iyo-gun, Ehime, Japan.
Introduction:
This study evaluated the immunocytochemical (ICC) expression of IMP3 in direct endometrial brushings processed as liquid-based cytology (LBC) samples of endometrioid adenocarcinoma (EAC), serous carcinoma (ESC) and surface papillary syncytial change (SPSC) with endometrial glandular and stromal breakdown (EGBD) to exploit its possible differential diagnostic aid.
Methods:
In total, 333 samples of LBC samples were obtained from selected outpatients in parallel with Pipelle endometrial sampling. They consisted of 97 EAC (83 grade 1: EAC-1, 14 EAC-3), 35 ESC and 201 benign endometrial samples (51 proliferative, 42 secretory, 38 atrophic, 70 SPSC with EGBD). ICC expression of insulin-like growth factor-II mRNA-binding protein 3 (IMP3) was manually performed on Papanicolaou-stained LBC samples.
Results:
The ESC samples showed positive staining cells in 100%, EAC-3 in 28.5%, and EAC-1 in 2.4% cases. All the benign endometrium samples were negative. Only ESC cases showed strong immunoreactivity (≥3+) in more than 50% of tumour cells with an average frequency of 80%.
Conclusions:
IMP3 is a helpful immunomarker to distinguish ESC from EAC and SPSC in endometrial cytology.
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