Expression of CaMV ORF IV in Escherichia coli

A CaMV DNA fragment corresponding to nucleotides 2200-3992 and including the coding sequence (2200-3670) of open reading frame IV was inserted in the pTG908 prokaryotic expression vector. In the recombinant pTG-IV plasmid, ORF IV, which codes for the coat protein precursor, was fused to the N-terminal coding sequence of the lambda CII gene, which is under transcriptional control of the lambda PL promoter. The expected fusion protein CII-ORF IV had a calculated molecular weight of 58.4 Kd. Nevertheless, temperature induction of the PL promoter resulted in synthesis of a major 76-Kd fusion protein: the coat protein precursor migrated abnormally on SDS polyacrylamide gel.