Search research articles

Search research articles

Related Experiment Videos

Mesodermal cell lineage determination and proto-oncogene expression.

D A Ketelaar¹, G R Utesch, E Sierra

¹Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.

Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists

|November 1, 1988

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Morphological and molecular characterization of Crassicauda anthonyi in Cuvier's beaked whales from the Canary Islands.

BMC veterinary research·2025

Same author

Seminoma in an adult striped dolphin Stenella coeruleoalba: tomographic, macroscopical, histological and immunohistochemical study.

Diseases of aquatic organisms·2020

Same author

Advances in research on the impacts of anti-submarine sonar on beaked whales.

Proceedings. Biological sciences·2019

Same author

Retrospective study of foreign body-associated pathology in stranded cetaceans, Canary Islands (2000-2015).

Environmental pollution (Barking, Essex : 1987)·2018

Same author

Deadly acute Decompression Sickness in Risso's dolphins.

Scientific reports·2017

Same author

Coinfection by Streptococcus phocae and cetacean morbillivirus in a short-beaked common dolphin Delphinus delphis.

Diseases of aquatic organisms·2017

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

A rat embryo fibroblastic cell line spontaneously differentiates into muscle and fat cells. Researchers found no significant changes in proto-oncogene RNA levels during this mesodermal lineage determination.

Area of Science:

Cell Biology
Developmental Biology
Molecular Biology

Background:

Established an immortal, nontumorigenic rat embryo fibroblastic cell line with a normal diploid karyotype.
This cell line serves as a valuable model for studying cellular differentiation and determination.

Purpose of the Study:

To investigate the role of proto-oncogenes in mesodermal lineage determination.
To characterize preadipocytes and myoblasts derived from the established cell line.
To explore the molecular basis of cell lineage determination using a normal karyotype model system.

Main Methods:

Spontaneous differentiation of the rat embryo fibroblastic cell line into myotubes and adipocytes.
Isolation and characterization of lineage-determined (nondifferentiated) preadipocytes and myoblasts.

Related Experiment Videos

Analysis of proto-oncogene RNA expression levels during lineage determination.

Main Results:

The rat embryo fibroblastic cell line successfully differentiated into mesodermal cell lineages, forming myotubes and adipocytes.
No significant alterations in the RNA levels of 15 tested proto-oncogenes were observed correlating with mesodermal lineage determination.
Isolated preadipocyte and myoblast cell lines confirmed lineage-specific differentiation potential.

Conclusions:

The 15 proto-oncogenes examined do not appear to be involved in the mesodermal lineage determination process.
Further investigation is warranted to explore other potential proto-oncogenes or molecular mechanisms in cell lineage determination.
The established cell lines provide an excellent model for future studies on the molecular underpinnings of cell differentiation.