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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
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A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples.

Axelle Kerstens1,2,3, Nikky Corthout1,2,3, Benjamin Pavie1,2,3

  • 1VIB Bio Imaging Core, Herestraat 49, Box 602, 3000, Leuven, Belgium.

BMC Biology
|January 9, 2019
PubMed
Summary

A new optical imaging method, ALMOST, enables 3D surface visualization of opaque biological samples. This technique overcomes limitations of current methods, allowing detailed surface morphology assessment of non-transparent specimens like Drosophila and Xenopus embryos.

Keywords:
3D imagingLabel-freeMesoscaleMorphologyOpaque samplesReal colorTomography

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Area of Science:

  • Biomedical Optics
  • Developmental Biology
  • Imaging Science

Background:

  • Current mesoscale 3D imaging is limited to transparent samples or X-rays.
  • Opaque samples like Drosophila and Xenopus embryos lack 3D surface morphology assessment.
  • A novel optical method, ALMOST, is developed for opaque sample imaging.

Purpose of the Study:

  • To develop a novel optical method for 3D surface imaging of reflective opaque objects.
  • To enable 3D color surface morphology assessment of non-transparent biological samples.
  • To overcome limitations of existing mesoscale 3D imaging techniques.

Main Methods:

  • Utilized an optical projection tomography device with oblique illumination and optical filters.
  • Developed the ALMOST (Advanced Light-enabled Mesoscopic Optical Surface Tomography) method.
  • Employed filtered back projection algorithm and 3D software for reconstruction and rendering.
  • Expanded to fluorescence and multi-channel spectral imaging, validated with micro-computed tomography.

Main Results:

  • Demonstrated image formation and reconstruction for opaque samples.
  • Showcased versatility with various biological and inorganic test samples.
  • Applied ALMOST to study Drosophila larval form changes, adult Drosophila imaging, and live Xenopus embryo neurulation.
  • Validated ALMOST against transmitted light and fluorescence optical projection tomography.

Conclusions:

  • ALMOST provides spectral/color, macro/mesoscopic 3D imaging for diverse model organisms.
  • Enables longitudinal surface dynamics visualization during development.
  • Offers a new modality for studying non-transparent biological samples in 3D.