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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: December 22, 2020
Extracellular Vesicles Released by Human Induced-Pluripotent Stem Cell-Derived Cardiomyocytes Promote Angiogenesis.
Julie A Dougherty1,2, Naresh Kumar1,2, Mohammad Noor1,2
1Dorothy M. Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
Extracellular vesicles (EVs) from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) promote new blood vessel growth (angiogenesis). This cell-free therapy shows potential for treating heart damage after myocardial infarction.
Area of Science:
- Cardiovascular Research
- Regenerative Medicine
- Cell Biology
Background:
- Stem cell therapy for myocardial repair relies on paracrine signaling.
- Extracellular vesicles (EVs) from stem cells stimulate angiogenesis and cytoprotection.
- The angiogenic potential of EVs from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) is unknown.
Purpose of the Study:
- To isolate, characterize, and evaluate the in vitro angiogenic potential of EVs from hiPSC-CM conditioned media.
Main Methods:
- hiPSC-CM cultured for 2 weeks; EVs isolated from conditioned media.
- EVs characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis, and immunoblotting.
- Angiogenic potential assessed using tube formation, wound-healing, and proliferation assays in bovine aortic endothelial cells (BAEC). Gene expression of growth factors in hiPSC-derived endothelial cells (hiPSC-EC) treated with EVs was analyzed.
Main Results:
- TEM and nanoparticle tracking confirmed EV presence and characteristics.
- Immunoblotting showed enrichment of EV markers (CD63, HSP70).
- BAEC treated with hiPSC-CM-derived EVs (CM-EVs) exhibited significantly increased tube formation, wound closure, and proliferation.
- CM-EV treatment induced increased expression of pro-angiogenic growth factors in hiPSC-EC.
Conclusions:
- EVs derived from hiPSC-CM enhance angiogenesis in endothelial cells.
- This acellular/cell-free approach offers a potential therapeutic strategy for inducing angiogenesis in myocardial infarction patients.
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