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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Accessory Structures of the Skin: Sweat Glands01:20

Accessory Structures of the Skin: Sweat Glands

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Sweat glands or sudoriferous glands are one of the important accessory structures of the skin. They are small, coiled tubular structures located in the dermis, the middle layer of the skin. Sweat glands are responsible for producing and secreting sweat, a watery fluid that helps regulate body temperature and excrete waste products.
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Capillary Beds01:20

Capillary Beds

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Capillary beds are networks of tiny blood vessels that play a crucial role in the circulatory system. These beds are where the exchange of gases, nutrients, and waste products occurs between the blood and surrounding tissues. Each capillary bed consists of numerous capillaries, which are the smallest blood vessels in the body, typically only one cell-thick. This thinness allows for the efficient diffusion of substances.
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Capillary Exchange01:28

Capillary Exchange

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The cardiovascular system's chief role is to disseminate gases, nutrients, waste, and other substances to the body's cells. Small molecules like gases, lipids, and lipid-soluble substances directly diffuse through capillary wall endothelial cell membranes. Glucose, amino acids, and ions, including sodium, potassium, calcium, and chloride, use transporters for facilitated diffusion via membrane-specific channels. Glucose, ions, and bigger molecules may also pass through intercellular...
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Roles of Electrolytes: Chloride and Bicarbonate01:29

Roles of Electrolytes: Chloride and Bicarbonate

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Chloride ions contribute to the osmotic pressure gradient distinguishing the intracellular fluid (ICF) from the extracellular fluid (ECF). They counterbalance positively charged ions in the ECF and ensure its electrochemical stability. The renal system's process of chloride absorption and release generally mirrors that of sodium ions.
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Related Experiment Video

Updated: Jan 31, 2026

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Sweat chloride quantification using capillary electrophoresis.

Patricia Dubot1, Jing Liang2, Jacobé Dubs2

  • 1Laboratoire de Biochimie, Institut Fédératif de Biologie, Hôpital Purpan, 330, av de Grande Bretagne TSA 40031, 31059 Toulouse Cedex 9, France.

Practical Laboratory Medicine
|January 10, 2019
PubMed
Summary
This summary is machine-generated.

The WynSep capillary electrophoresis method accurately measures sweat chloride for cystic fibrosis (CF) diagnosis, offering a fast and easy alternative to traditional sweat tests.

Keywords:
CE, capillary electrophoresisCF, cystic fibrosisCFTR, cystic fibrosis transmembrane conductance regulatorCV, coefficient of variationCapillary electrophoresisChlorideCystic fibrosisIS, internal standardLOD, limit of detectionST, sweat testSweat test

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Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Genetics

Background:

  • Cystic fibrosis (CF) is a severe genetic disorder prevalent in European populations.
  • Diagnosis relies on elevated sweat chloride levels measured via sweat testing (ST).
  • WynSep developed a capillary electrophoresis with contactless conductivity detection (CE-C4D) for low-volume sweat chloride analysis.

Purpose of the Study:

  • Evaluate the clinical feasibility of the WynSep-CE device for CF diagnosis.
  • Compare the WynSep-CE method against the standard coulometric sweat chloride measurement.

Main Methods:

  • Analyzed 65 patient sweat samples using both coulometric and WynSep-CE methods.
  • Performed method validation and comparative statistical analyses (Bland-Altman, Passing-Bablok).

Main Results:

  • 53 samples were analyzed by both methods.
  • WynSep-CE demonstrated comparable analytical performance to the coulometric method.
  • High correlation (r=0.993) and close agreement were observed, with no false positives or negatives.

Conclusions:

  • WynSep-CE is an accurate and reliable method for sweat chloride analysis.
  • The device is fast, easy-to-use, and suitable for cystic fibrosis diagnosis.
  • This method offers a viable alternative for biochemical CF diagnosis.