Related Experiment Video

Updated: Jan 31, 2026

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
09:24

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Published on: May 9, 2020

4.8K

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway

Miaomiao Ge1, Lu Zhang1, Lina Cao1

  • 1a School of Public Health , Xuzhou Medical University , Xuzhou , China.

International Journal of Food Sciences and Nutrition
|January 10, 2019
PubMed

Insights

Sulforaphane (SFN), a broccoli extract, inhibits gastric cancer by targeting cancer stem cells (CSCs). It suppresses the Sonic hedgehog (Hh) pathway, crucial for CSC stemness, offering a promising avenue for gastric cancer intervention.

Area of Science:

  • Oncology
  • Molecular Biology
  • Natural Products Chemistry

Background:

  • Gastric cancer is a prevalent malignancy globally.
  • Sulforaphane (SFN), derived from broccoli, exhibits known anti-cancer properties.
  • Cancer stem cells (CSCs) play a critical role in gastric cancer development and resistance to therapy.

Purpose of the Study:

  • To investigate the inhibitory effects of Sulforaphane (SFN) on gastric cancer.
  • To elucidate the role of the Sonic hedgehog (Hh) pathway in SFN's anti-cancer activity.
  • To evaluate SFN's impact on gastric cancer stem cells (CSCs).

Main Methods:

  • Treatment of gastric cancer cells with Sulforaphane (SFN).
  • Assessment of tumorsphere formation and CSC marker expression.
  • Analysis of proliferation, apoptosis, and Sonic Hh pathway activation.

Main Results:

  • SFN significantly reduced tumorsphere formation and CSC marker expression.
  • SFN suppressed proliferation and induced apoptosis in gastric CSCs.
  • SFN inhibited Sonic Hh pathway activation, which was crucial for maintaining CSC stemness.

Conclusions:

  • Sulforaphane (SFN) demonstrates potent anti-cancer activity against gastric cancer by targeting CSCs.
  • Suppression of the Sonic Hh pathway is a key mechanism underlying SFN's efficacy.
  • SFN represents a promising natural compound for gastric cancer intervention.
Keywords:
Sonic hedgehog pathwaySulforaphanegastric cancer stem cellssuppression

More Related Videos

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

1.1K
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.7K

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.5K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.8K
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K