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Related Experiment Video

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Xenopus slc7a5 is essential for notochord function and eye development.

Tomohisa Katada1, Hiroyuki Sakurai1

  • 1Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka, Tokyo 181-8611, Japan.

Mechanisms of Development
|January 10, 2019
PubMed
Summary

The amino acid transporter SLC7A5 (LAT1) is crucial for early development in Xenopus embryos, impacting notochord and eye formation independently of its transport function.

Keywords:
Amino acid transporterNeurogenesisSonic hedgehogXenopus laevisslc7a5

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Solute carrier family 7 member 5 (SLC7A5), also known as LAT1, is recognized for its role in amino acid transport.
  • Previous studies indicate SLC7A5 involvement in cancer and development, but its precise function in early embryogenesis is unclear due to embryonic lethality in mice.
  • Xenopus laevis embryos offer a model to study placental-independent developmental processes.

Purpose of the Study:

  • To investigate the expression and function of SLC7A5 in early Xenopus laevis development.
  • To elucidate the role of SLC7A5 in neural patterning, neurogenesis, and eye development.
  • To explore the potential involvement of SLC7A5 in the sonic hedgehog (Shh) signaling pathway during development.

Main Methods:

  • Expression analysis of slc7a5 in Xenopus laevis embryos.
  • Loss-of-function studies using morpholino antisense oligonucleotides targeting slc7a5.
  • Assessment of neural and non-neural patterning, primary neurogenesis, eye development, and Shh signaling pathway activity.

Main Results:

  • SLC7A5 expression was detected in the notochord and eye prior to the late neurula stage.
  • Inhibition of slc7a5 resulted in defects in neural and non-neural patterning, primary neurogenesis, and eye development.
  • Impaired notochord development and compromised Shh/Gli signaling were observed in slc7a5-inhibited embryos.

Conclusions:

  • SLC7A5 is essential for notochord development and subsequent primary neurogenesis, mediated through the Shh/Gli signaling pathway.
  • SLC7A5 plays a critical role in eye development.
  • These developmental functions of SLC7A5 appear to be independent of its canonical amino acid transport activity in early embryonic stages.