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Updated: Jan 31, 2026
08:42
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
Published on: April 10, 2019
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[Pyroptosis is involved in the pathogenesis of necrotizing enterocolitis]
1Department of Neonatology, First Affiliated Hospital, University of South China, Hengyang 421001, China. *Corresponding author,
Summary
Pyroptosis, a form of programmed cell death, plays a role in necrotizing enterocolitis (NEC) pathogenesis. This study found increased pyroptosis markers in NEC rat models, suggesting its involvement in the disease process.
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease affecting newborns.
- The precise mechanisms underlying NEC pathogenesis require further elucidation.
Purpose of the Study:
- To investigate the role of pyroptosis in the pathogenesis of necrotizing enterocolitis (NEC).
Main Methods:
- A rat model of NEC was established using hypoxia and cold stimulation.
- Histopathological examination (HE staining) of ileocecal tissues was performed.
- Levels of pyroptosis-related markers (IL-18, IL-1β, NLRP3, caspase-1) were quantified using RT-qPCR, ELISA, and Western blot.
Main Results:
- NEC group rats exhibited poor general condition and significant intestinal epithelial damage.
- Elevated mRNA and protein levels of IL-18, IL-1β, and NLRP3 were observed in NEC tissues.
- Caspase-1 levels were reduced, while pyroptosis executioner proteins (P10, P20) were present in NEC tissues.
Conclusions:
- Pyroptosis is implicated in the pathogenesis of necrotizing enterocolitis.
- These findings highlight pyroptosis as a potential therapeutic target for NEC.
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