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Updated: Jan 30, 2026

Pancreatic Islet Embedding for Paraffin Sections
Published on: June 29, 2018
Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective
Matthew J Powell-Palm1, Yanfang Zhang2, Justin Aruda1
1Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Abstract:
While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.
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