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Extractive Fermentation for Process integration and amplified pullulan production by A. pullulans in Aqueous Two
Parul Badhwar1, Punit Kumar1, Kashyap Kumar Dubey2
1Microbial Process Development Laboratory University Institute of Engineering and Technology Maharishi Dayanand University, Rohtak, 124001, Haryana, India.
Scientific Reports
|January 12, 2019
Summary
Extractive fermentation using Aqueous Two Phase Systems (ATPS) enhances pullulan yield. This novel approach integrates production and recovery, achieving a maximum yield of 36.47 g/L for economic and efficient pullulan manufacturing.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Industrial Microbiology
Background:
- Extractive fermentation, or in situ product recovery, offers a novel approach for simultaneous product separation during fermentation.
- Traditional fermentation processes can be limited by product inhibition and complex downstream processing.
- Optimizing pullulan production is crucial for its diverse industrial applications.
Purpose of the Study:
- To apply extractive fermentation using Aqueous Two Phase Systems (ATPS) for economic and high-yield production of pullulan from A. pullulans.
- To design and evaluate ATPS with varying polyethylene glycol (PEG) molecular masses and phase-forming polymers (dextran or phosphate salts).
- To determine the optimal ATPS composition for maximizing pullulan yield and facilitating simultaneous recovery.
Main Methods:
- Designed ATPS using different molecular masses of PEG (400, 600, 4000, 6000) with dextran or sodium phosphate salts.
- Selected systems with short Tie Line Length (TLL) and maintained a constant volume ratio of 1.0.
- Optimized pH for different ATPS compositions (pH 7.0 for PEG-dextran and PEG-NaH2PO4; pH 9.0 for PEG-Na2HPO4).
- Assessed the viability of A. pullulans in selected ATPS and determined phase partitioning of biomass and pullulan.
Main Results:
- A. pullulans remained viable in PEG-NaH2PO4 and PEG-dextran ATPS.
- Biomass preferentially partitioned to the PEG-rich top phase, while pullulan concentrated in the bottom phase.
- A maximum pullulan yield of 36.47 g/L was achieved using a PEG 4000-Dextran 500 system.
- The extractive fermentation process integrated upstream and downstream operations, reducing overall process time.
Conclusions:
- Extractive fermentation with ATPS is an effective strategy for enhancing pullulan yield and simplifying recovery.
- The PEG 4000-Dextran 500 system demonstrated superior performance for simultaneous pullulan production and separation.
- This integrated approach offers a more efficient and economic method for continuous pullulan manufacturing.
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