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Updated: Jan 30, 2026

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Hepatocyte Growth Factor-Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKD

Masatoshi Oka1,2, Sachiko Sekiya2, Ryoichi Sakiyama3

  • 1Department of Medicine, Kidney Center.

Journal of the American Society of Nephrology : JASN
|January 13, 2019
PubMed

Insights

Cell sheet therapy using hepatocyte growth factor (HGF) transgenic mesothelial cells shows promise for treating kidney fibrosis. This novel approach effectively suppressed fibrosis and maintained renal blood flow in preclinical models.

Area of Science:

  • Nephrology
  • Regenerative Medicine
  • Biotechnology

Background:

  • Hepatocyte growth factor (HGF) exhibits antifibrotic, angiogenic, and vasodilatory properties.
  • Systemic HGF administration is not a viable strategy for managing chronic kidney disease (CKD) interstitial fibrosis.
  • Novel therapeutic approaches are needed to combat progressive kidney fibrosis.

Purpose of the Study:

  • To investigate the efficacy of HGF-transgenic mesothelial cell sheet therapy for kidney fibrosis.
  • To compare HGF-transgenic cell sheets with other interventions in a rat model of kidney fibrosis.

Main Methods:

  • Human mesothelial cells genetically engineered to produce HGF were cultured into cell sheets.
  • Rats with unilateral ureteral obstruction (UUO) underwent transplantation of HGF-transgenic cell sheets to the kidney surface.
  • Interventions included HGF-transgenic cell sheets (kidney or thigh), non-transgenic cell sheets (kidney), no sheets, or HGF injections.

Main Results:

  • HGF-transgenic cell sheets transplanted to the kidney significantly suppressed myofibroblast induction and collagen deposition for 28 days.
  • Other tested interventions did not demonstrate significant antifibrotic effects.
  • HGF-secreting cell sheets preserved peritubular capillaries and maintained renal blood flow.

Conclusions:

  • Cell sheet therapy utilizing HGF-transgenic mesothelial cells represents a novel and promising strategy for inhibiting progressive kidney fibrosis.
  • This approach holds potential for therapeutic applications in chronic kidney disease (CKD).
Abstract

Keywords:
HGFcell sheetchronic kidney diseasefibrosisobstructive nephropathy

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