Related Experiment Video
Updated: Jan 30, 2026

Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
Application of real-time PCR for tree nut allergen detection in processed foods
Rosario Linacero1, Africa Sanchiz2, Isabel Ballesteros3
1Departamento de Genética, Fisiología y Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain.
Abstract:
Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
The Tree of Life - Bacteria, Archaea, Eukaryotes
Real-World Application of Classical Conditioning
Higher-order, or second-order, conditioning occurs when a neutral stimulus becomes associated with an already established conditioned stimulus through repeated pairings. For instance, if a dog has been...
PCR
Survival Tree
Building a Survival Tree
Constructing a...
Phylogenetic Trees

