Related Experiment Video

Updated: Jan 30, 2026

Mitochondrial Respiration Quantification in Yeast Whole Cells
07:15

Mitochondrial Respiration Quantification in Yeast Whole Cells

Published on: November 8, 2024

1.3K

Srv2 Is a Pro-fission Factor that Modulates Yeast Mitochondrial Morphology and Respiration by Regulating Actin

Ying-Chieh Chen1, Tzu-Hao Cheng2, Wei-Ling Lin1

  • 1Institute of Biotechnology, National Tsing Hua University, Life Science Building II, Room 506 No. 101, Section 2, Kuang-Fu Road, Hsin Chu City 30013, Taiwan, ROC.

Iscience
|January 15, 2019
PubMed

Insights

The actin-regulatory protein Srv2/CAP interacts with mitochondrial fission GTPase Dnm1/DRP1. Deleting Srv2 disrupts mitochondrial fission, leading to elongated mitochondria and reduced respiration.

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Actin Dynamics

Background:

  • Mitochondrial dynamics, including fusion and fission, are crucial for cellular organelle regulation and function.
  • Disruptions in mitochondrial dynamics are linked to neurodegenerative diseases.
  • While proteins directly mediating mitochondrial fusion/fission are well-studied, regulatory machineries remain less explored.

Purpose of the Study:

  • To investigate the role of actin-regulatory proteins in mitochondrial dynamics.
  • To identify novel regulators of mitochondrial fission and function.

Main Methods:

  • Yeast genetics and cell biology techniques.
  • Mitochondrial morphology analysis.
  • Respiration assays.
  • Protein interaction studies.

Main Results:

  • Identified an interaction between Dnm1/DRP1 (mitochondrial fission GTPase) and Srv2/CAP (actin-regulatory protein) at mitochondria.
  • Deletion of Srv2 in yeast resulted in elongated, hyperfused mitochondria.
  • Srv2 deletion led to reduced reserved respiration capacity and disrupted mitochondrial actin assembly.

Conclusions:

  • Srv2/CAP acts as a pro-fission factor in regulating mitochondrial dynamics.
  • Srv2 influences mitochondrial morphology and activity through its actin-regulatory functions.
  • This study reveals a novel link between actin regulation and mitochondrial fission.
Keywords:
Cell BiologyFunctional Aspects of Cell BiologyMolecular Biology

More Related Videos

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe08:07

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

935
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.7K

Related Concept Videos

Factors Affecting Respiration01:24

Factors Affecting Respiration

Respiration is a crucial physiological function involving exchanging oxygen (O2) and carbon dioxide (CO2) between an organism and its environment. Various factors can impact this essential process:
9.2K
Nuclear Fission02:50

Nuclear Fission

Many heavier elements with smaller binding energies per nucleon can decompose into more stable elements that have intermediate mass numbers and larger binding energies per nucleon—that is, mass numbers and binding energies per nucleon that are closer to the “peak” of the binding energy graph near 56. Sometimes neutrons are also produced. This decomposition of a large nucleus into smaller pieces is called fission. The breaking is rather random with the formation of a large...
12.5K
Other Factors Affecting Respiration Centers01:17

Other Factors Affecting Respiration Centers

Breathing is primarily an involuntary activity regulated by the brainstem respiratory centers. However, it can also be consciously controlled, allowing us to hold our breath or take deeper breaths when needed. This voluntary control is facilitated by the cerebral motor cortex, which bypasses the medullary centers to stimulate the respiratory muscles directly.
However, the ability to hold one's breath voluntarily is not limitless. When the CO2 concentration in the blood reaches a critical...
1.6K
Chemical Factors Affecting Respiration Centers01:31

Chemical Factors Affecting Respiration Centers

Chemical factors such as changing CO2, O2, and H+ levels in arterial blood play a critical role in influencing respiration depth and rates. These variations are detected by chemoreceptors—specialized sensors located in two primary body areas. Central chemoreceptors are found throughout the brain stem, including the ventrolateral medulla, while peripheral chemoreceptors are located in the aortic arch and carotid arteries.
CO2 has a potent influence on respiration and is strictly regulated....
2.4K
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Binary Fission01:20

Binary Fission

Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
63.3K