Related Experiment Video
Updated: Jan 30, 2026

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
Xiaoyun Fu1,2, Shelby A Cate3, Melissa Dominguez3
1Bloodworks Research Institute, Seattle, Washington, USA. xiaoyunf@bloodworksNW.org.
Abstract:
We developed a high-throughput mass spectrometry-based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment.
Related Concept Videos
Oxidation Numbers
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
Responses to Heat and Cold Stress
Responses to Salt Stress
Oxidation-Reduction Reactions
Oxidation of Alcohols
The process of oxidation in a chemical reaction is observed in any of the three forms:

