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Updated: Jan 30, 2026
08:41
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Published on: August 16, 2012
12.0K
Fast upconversion super-resolution microscopy with 10 μs per pixel dwell times.
Xingyun Peng1, Bingru Huang, Rui Pu
1Centre for Optical and Electromagnetic Research, South China Academy of Advanced Optoelectronics, South China Normal University, 510006 Guangzhou, China. zhanqiuqiang@m.scnu.edu.cn.
Nanoscale
|January 16, 2019
Summary
This study enhances multi-photon upconversion super-resolution microscopy by using a high Yb3+ doping strategy. This significantly boosts scanning speed for real-time biological imaging.
Area of Science:
- Biomedical imaging
- Nanotechnology
- Optical microscopy
Background:
- Multi-photon upconversion super-resolution microscopy offers advantages like deep tissue imaging and low phototoxicity.
- Current limitations include slow scanning speeds due to long lanthanide emission lifetimes and low brightness.
Purpose of the Study:
- To overcome the speed limitations of upconversion super-resolution microscopy.
- To enable faster imaging for capturing dynamic biological processes.
Main Methods:
- Developed a facile strategy using a high ytterbium (Yb3+) sensitizer doping approach for upconversion nanocrystals.
- Optimized nanocrystal properties to increase emission intensity and accelerate emission transients.
Main Results:
- Achieved a significant increase in upconversion nanocrystal brightness and a substantial reduction in emission lifetime.
- Enabled a 10 μs per pixel scanning speed for upconversion stimulated emission depletion super-resolution microscopy.
- Maintained emission depletion efficiency.
Conclusions:
- The high Yb3+ doping strategy effectively overcomes speed limitations in upconversion super-resolution microscopy.
- This advancement paves the way for real-time observation of biological activities using this imaging technique.
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