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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Range00:59

Range

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The range is one of the measures of variation. It can be defined as the difference between a dataset's highest and lowest values. For example, in the study of seven 16-ounce soda cans, the filled volume of soda was measured, thus producing the following amount (in ounces) of soda:
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Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
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Free space variable optical attenuator using frustrated total internal reflection with 70  dB dynamic range.

Tiberius S Georgescu, Ioan Dancus, Daniel Ursescu

    Applied Optics
    |January 16, 2019
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    Summary
    This summary is machine-generated.

    High power laser systems require adjustable light dimming. A novel system uses frustrated total internal reflection to achieve over 70 dB of continuous transmittance variation for laser power control.

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    Area of Science:

    • Optics and Photonics
    • Laser Technology

    Background:

    • High power laser systems necessitate precise control over light field intensity.
    • Existing methods like optical filters offer limited dynamic range and variability for laser attenuation.

    Purpose of the Study:

    • To develop a system for continuously variable light field power attenuation.
    • To overcome the limitations of fixed optical filters in high dynamic range applications.

    Main Methods:

    • Utilized the principle of frustrated total internal reflection (TIR).
    • Designed a system to modulate light transmittance dynamically.

    Main Results:

    • Demonstrated continuous variation of transmittance over a 70-decibel (dB) dynamic range.
    • Achieved adjustable laser power dimming exceeding conventional methods.

    Conclusions:

    • Frustrated total internal reflection provides a viable solution for high dynamic range laser power attenuation.
    • The developed system offers the required variability for advanced laser experiments.