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[SPECIFICITY EVALUATION OF TRCReady® MTB AND TRCReady® MAC FOR IDENTIFYING MYCOBACTERIUM TUBERCULOSIS COMPLEX,
Abstract:
[Objective] To evaluate the specificity of TRC- Ready® MTB and TRCReady® MAC (Tosoh Bioscience, Japan) for identifying M.tubercidosis complex (MTC), M.avium and M. intracellulare. [Method] We tested TRCReady® MTB and TRCReady® MAC using TRCReady®-80 (Tosoh Bioscience, Japan), which is an automated nucleic amplification test instrument, with 151 Mycobacterium species (4 MTC and 147 Non-tuberculosis Mycobacterium (NTM) type strains). [Results] The specificity of TRCReady® MTB was 100%, however, TkCReady® MAC misidentified a total of six NTMs, M.arosiense, M.chimaera, M.colombiense, M.marseillense, M. vulneris and M.yongonense, as M. intracellulare. Then, the specificity for TRCReady® MAC was 96.0% (145/151). [Discussion] TRCReady® MTB and TRCReady® MAC are highly specific for identifying MTC, M.avium and M. intracellulare. Six NTM species which have been rarely isolated in Japan showed false-positive results as M.intra- celludare. However, when a sample was identified as M.in- tracellulare, the phenotypic characteristics like colony mor- phology would be carefully examined.
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