Related Experiment Video
Updated: Jan 30, 2026
06:40
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
Published on: December 28, 2021
4.0K
A novel screening method for potential naringinase-producing microorganisms
Satish V Patil1,2, Sunil H Koli1, Bhavana V Mohite1
1School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.
Biotechnology and Applied Biochemistry
|January 17, 2019
Summary
A new, simple plate assay using iodine vapors allows for effective screening of microorganisms for naringinase production. This method addresses a key research gap, enabling the discovery of more potent naringinase enzymes for industrial applications.
Area of Science:
- Biotechnology
- Enzyme Technology
- Microbiology
Background:
- Naringinase is crucial for various industrial applications, but research progress is hindered by a lack of efficient screening methods.
- Existing screening methods are often complex or not universally applicable across different microbial types (bacteria, fungi, actinomycetes).
Purpose of the Study:
- To develop a simple, effective, and broadly applicable plate assay for screening microorganisms that produce naringinase.
- To overcome the limitations of current screening techniques and accelerate the discovery of novel naringinase enzymes.
Main Methods:
- Development of a novel plate assay utilizing iodine vapor exposure.
- Application of the assay for screening diverse microbial populations for naringinase activity.
Main Results:
- The developed plate assay provides a simple and effective method for identifying naringinase-producing microorganisms.
- This technique is applicable across different microbial genera, including bacteria, fungi, and actinomycetes.
Conclusions:
- The new iodine-based plate assay effectively fills a technological gap in naringinase research.
- This method facilitates the screening of a wider range of microorganisms, potentially leading to the identification of superior naringinase producers for industrial use.
Related Concept Videos
01:29Types of Microorganisms
2.2K
Microorganisms are a diverse group of microscopic entities broadly categorized into cellular and acellular types based on their structural organization. Cellular microorganisms include bacteria, archaea, fungi, protozoa, and algae, while acellular microorganisms are represented by viruses.Cellular MicroorganismsBacteriaBacteria, tiny prokaryotic organisms, exhibit fascinating shapes such as rods, spheres, and spirals. They adapt to diverse habitats, including soil, water, and human-associated...
2.2K
01:18Mutations in Microorganisms
723
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
723
01:30Environmental Applications of Microorganisms
1.1K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.1K
01:29Microorganisms in Medicine and Therapeutics
1.1K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.1K
01:27Microorganisms in Agriculture and Food industry
1.5K
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
1.5K
01:17Controlled-Potential Coulometry: Electrolytic Methods
716
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
The chosen potential...
716