Related Experiment Video
Updated: Jan 30, 2026

Generation of Human Chimeric Antigen Receptor Regulatory T Cells
Published on: January 3, 2025
Identification and Isolation of Regulatory B Cells in Mouse and Human
Madhvi Menon1, Elizabeth C Rosser1, Claudia Mauri2
1Centre for Rheumatology, Division of Medicine, University College London, 5 University Street, London, WC1E 6JF, UK.
Abstract:
Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.
Related Concept Videos
Cis-regulatory Sequences
Cis-regulatory Sequences
Global Regulatory Systems
Drug Control Governance: Regulatory Bodies and Their Impact
Methods of Classification and Identification
Overview Of Cell Separation And Isolation

