Related Experiment Video
Updated: Jan 30, 2026

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
Development of real-time RT-PCR assays for two viruses infecting pome fruit
E Beaver-Kanuya1, S A Szostek1, S J Harper1
1Department of Plant Pathology, Washington State University, Prosser, WA, 99350, United States.
Abstract:
Apple stem grooving virus (ASGV) and Apple green crinkle-associated virus (AGCaV) negatively impact production, maintenance, and distribution of apples and other Malus species world-wide. Due to the increasing diversity of isolates found by high-throughput sequencing, we have developed real-time RT-qPCR assays for these two viruses. Primers and probes were designed against alignments of representative extant sequences from around the world, and reaction conditions optimized for sensitivity and specificity. Assays were validated against a panel of virus isolates, and compared to extant endpoint RT-PCR and ELISA assays. The new real-time RT-qPCR assays showed greater detection sensitivity than extant assays and were able to detect their target viruses from different host tissues.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Fruit Development, Structure, and Function
PCR
What are Viruses?
Real Number System
Sustainable Development

