Related Experiment Video
Updated: Jan 30, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA
Luis E Valencia1, Zhicheng Zhang, Alexis J Cepeda
1Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA. adriankc@utexas.edu.
Abstract:
Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.
Related Concept Videos
Intracellular Signaling Cascades
Rab Cascades
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
Cascaded Op Amps
In a cascaded system, each op-amp is referred to as a stage. The output of one stage drives the input of the subsequent stage. As the input signal passes through...

