A comprehensive and user-friendly framework for 3D-data visualisation in invertebrates and other organisms.

Area of Science:

• Biological Imaging
• Morphology
• Scientific Visualization

Background:

• 3D imaging techniques, such as X-ray micro-computed tomography (μCT), are increasingly accessible for biological research, particularly for small subjects like invertebrates.
• Current methods for visualizing and presenting 3D data lag behind the advancements in data acquisition, hindering broader adoption.
• Effective visualization is crucial for quantitative analysis of complex biological structures.

Purpose of the Study:

• To provide a comprehensive workflow for processing, visualizing, and presenting 3D imaging data.
• To encourage wider utilization of 3D imaging techniques in biological research, especially for invertebrates.
• To facilitate the creation of high-quality 3D figures for scientific publications.

Main Methods:

• Development of a detailed workflow for manipulating and visualizing 3D data from volume and surface-mesh renderings.
• Inclusion of basic and advanced options for generating various visual outputs, including images, videos, and interactive 3D-PDFs.
• Demonstration of visualization techniques for quantitative analysis of invertebrate morphology.

Main Results:

• A practical and accessible workflow for 3D data processing and visualization has been established.
• Examples of diverse 3D figure generation for publication are provided, showcasing different rendering and output options.
• The workflow supports the creation of high-resolution 3D figures suitable for modern scientific communication.

Conclusions:

• Implementing this workflow can simplify the use of 3D imaging data, thereby promoting its application in biological studies.
• Enhanced visualization methods, including interactive 3D-PDFs, improve the communication of scientific findings, particularly for microscopic invertebrates.
• The study advocates for the adoption of advanced 3D visualization techniques to advance morphological research and scientific illustration.