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Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells

Zhengfeng Xu1, Jiajun Wu1, Pan Cai1

  • 1Department of Orthopedics, Zhoupu Hospital Affiliated to Shanghai University of Medicine and Health Science, Shanghai 201318, P.R. China.

Oncology Letters
|January 19, 2019
PubMed

Insights

The fragile histidine triad (FHIT) gene inhibits osteosarcoma cell proliferation and promotes apoptosis. Overexpression of FHIT in Saos2 cells reversed the effects of FHIT deficiency, confirming its regulatory role.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • Osteosarcoma is a common primary bone malignancy.
  • The fragile histidine triad (FHIT) gene's role in osteosarcoma is not fully understood.
  • FHIT is a tumor suppressor gene involved in cell cycle regulation and apoptosis.

Purpose of the Study:

  • To investigate the regulatory effects of the fragile histidine triad (FHIT) gene on the proliferation and apoptosis of osteosarcoma cells.
  • To determine the impact of FHIT gene expression on Saos2 osteosarcoma cell behavior.

Main Methods:

  • Osteosarcoma Saos2 cells and hFOB1.19 cells were cultured.
  • Transfection with pcDNA3.1-FHIT overexpression vectors was performed.
  • FHIT mRNA and protein expression were analyzed using RT-qPCR and Western blot.
  • Cell proliferation was assessed using CCK8 assay, and apoptosis was detected by flow cytometry.

Main Results:

  • Saos2 cells showed decreased FHIT mRNA and protein expression compared to hFOB cells.
  • Transfection with FHIT vectors significantly increased FHIT mRNA and protein levels in Saos2 cells.
  • FHIT deficiency in Saos2 cells led to increased proliferation and decreased apoptosis.
  • FHIT overexpression in Saos2 cells inhibited proliferation and promoted apoptosis.

Conclusions:

  • The fragile histidine triad (FHIT) gene plays a crucial role in regulating osteosarcoma cell proliferation and apoptosis.
  • FHIT acts as a tumor suppressor by inhibiting proliferation and promoting apoptosis in Saos2 osteosarcoma cells.
  • FHIT gene therapy holds potential for treating osteosarcoma.
Keywords:
apoptosiscell proliferationfragile histidine triad geneosteosarcoma

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