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Related Experiment Videos

[Human leukocyte elastase].

J G Bieth1

  • 1INSERM U 237, Université Louis-Pasteur de Strasbourg, Faculté de Pharmacie, Illkirch, France.

Pathologie-Biologie
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

Human leucocyte elastase is a key enzyme in tissue remodeling and inflammation. Inhibitors are being developed to treat diseases like emphysema, where its role is most understood.

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Area of Science:

  • Biochemistry
  • Enzymology

Context:

  • Human leucocyte elastase (HLE) is a 30 kDa serine proteinase.
  • It degrades extracellular matrix proteins and plasma proteins.
  • HLE plays roles in neutrophil functions and tissue remodeling.

Purpose:

  • To review the properties of human leucocyte elastase.
  • To discuss its physiological and pathological roles.
  • To explore natural and synthetic inhibitors.

Summary:

  • HLE cleaves proteins at specific amino acid bonds (Val-X, Ala-X, Leu-X, Met-X).
  • Synthetic substrates offer a convenient method for measuring elastase activity.
  • Numerous natural inhibitors (e.g., alpha 1-antitrypsin) and synthetic inhibitors exist.

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Impact:

  • HLE is implicated in pulmonary emphysema, rheumatoid arthritis, and inflammation.
  • Understanding HLE is crucial for developing treatments for these conditions.
  • Inhibitor efficiency depends on association rates and complex stability.