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Endothelial progenitor cells and vascular endothelial growth factor in patients with Takayasu's arteritis
Luiz Samuel Gomes Machado1, Ana Cecilia Diniz Oliveira2, Patricia Semedo-Kuriki2
1Rheumatology Division, Escola Paulista de Medicina UNIFESP (Universidade Federal de São Paulo), Rua Doutor Diogo de Faria, 561, apt 12, Vila, Clementin São Paulo-SP, CEP: 04037-000, Brazil. luizmachado67@gmail.com.
Background:
Endothelial progenitor cells (EPCs) are responsible for endothelial damage repair. Takayasu's arteritis (TA) is a chronic inflammatory disease that affects large vessels. The aim of the study was to evaluate the number of EPCs and the levels of vascular endothelial growth factor (VEGF) and the relationship of these variables in patients with TA.
Methods:
Thirty women with TA and 30 healthy controls were included. EPCs were assessed by flow cytometry and cell culture and VEGF quantification was performed by commercial ELISA kits.
Results:
Ages of patients and controls were similar. The number of EPCs in patients and controls (median (interquartile range) were 0.0073% (0.0081%) vs. 0.0062% (0.0089%), p = 0.779 by flow cytometry and 27.0 (42.3) colony forming units (CFUs) vs. 27.0 (20.5) CFUs, p = 0.473 by cells culture, respectively. VEGF levels in patients and controls was 274.5 (395.5) pg/ml vs. 243.5 (255.3) pg/ml, p = 0.460. There was no difference in the number of EPCs and VEGF level between patients with active and inactive disease. There was a tendency of the number of angioblast-like EPCs in patients taking anti-TNFs to be higher; and in patients using methotrexate to be lower.
Conclusion:
No significant difference was found in the quantification of EPCs and VEGF levels in TA patients compared to controls, and no difference was observed between patients with active and inactive disease.