Related Experiment Video

Updated: Jan 30, 2026

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
09:16

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

10.7K

Generation of NERCe003-A-3, a p53 compound heterozygous mutation human embryonic stem cell line, by CRISPR/Cas9

Yingkang Li1, Chuan Huang2, Lagabaiyila Zha3

  • 1Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.

Stem Cell Research
|January 19, 2019
PubMed

Insights

Researchers created a p53 knockout human embryonic stem cell (hESC) line to study the gene's role. The p53-deficient hESCs maintained pluripotency and differentiation potential, offering insights into cancer development.

Area of Science:

  • Stem cell biology
  • Cancer genetics
  • Molecular biology

Background:

  • p53 is a crucial tumor suppressor gene regulating cell cycle, DNA repair, and senescence.
  • Alterations in p53 are common in human cancers, but its role in human embryonic stem cells (hESCs) remains unclear.

Purpose of the Study:

  • To investigate the consequences of p53 depletion in hESCs.
  • To generate and characterize a p53 knockout hESC line.

Main Methods:

  • CRISPR/Cas9 gene editing was used to create a p53 knockout hESC line (NERCe003-A-3) from a normal hESC line (NERCe003-A).
  • Karyotyping, assessment of pluripotency markers, and in vitro differentiation assays were performed.

Main Results:

  • The generated p53 knockout hESC line maintained a normal 46, XY karyotype.
  • Cells expressed pluripotency-related markers.
  • The p53-deficient hESCs demonstrated the capacity for in vitro differentiation into all three germ layers.

Conclusions:

  • p53 is not essential for maintaining pluripotency or differentiation potential in hESCs.
  • This p53 knockout hESC line serves as a valuable tool for studying p53 function and its implications in cancer biology.

More Related Videos

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

4.9K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.8K

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.8K
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.0K
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
Mutations01:39

Mutations

Overview
94.5K