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Updated: Jan 30, 2026

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
Characterization of binding interactions of anthraquinones and bovine β-lactoglobulin
Haoxie Xu1, Yuqin Lu1, Tingting Zhang1
1School of Medicine, Shenzhen University, Shenzhen, Guangdong Province 518060 PR China.
Abstract:
Anthraquinones, a class of naturally occurring polyphenolic compounds, exhibit a wide range of bioactivities. However, most free anthraquinones are lipophilic bioactive compounds. Bovine β-lactoglobulin (βLG), a major whey protein, has a high affinity for small hydrophobic compounds. In this study, the interactions between anthraquinones (rhein, emodin, and chrysophanol) and βLG were investigated by using fluorescence, circular dichroism (CD), Fourier-transform infrared spectroscopy (FTIR), and docking studies. These anthraquinones bound to the site near Trp19-Arg124 on βLG with a binding constant (Ka) between 103 and 105 L mol-1 to form complexes, which changed the secondary structure of βLG, inducing an α-helix to β-sheet structure transition. The order of binding increased with an increasing polarity in the order of rhein > emodin > chrysophanol. In addition, the degree of radical scavenging capacity masking increased with an increasing binding affinity. Complexation with βLG significantly increases the hydrosolubility of anthraquinones.
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