Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.5K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.5K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.5K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.5K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.0K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.0K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.7K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.7K
What is an Electrochemical Gradient?01:26

What is an Electrochemical Gradient?

127.8K
Adenosine triphosphate, or ATP, is considered the primary energy source in cells. However, energy can also be stored in the electrochemical gradient of an ion across the plasma membrane, which is determined by two factors: its chemical and electrical gradients.
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
127.8K
Gravitational Potential Energy for Extended Objects01:07

Gravitational Potential Energy for Extended Objects

2.0K
Consider a system comprising several point masses. The coordinates of the center of mass for this system can be expressed as the summation of the product of each mass and its position vector divided by the total mass:
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guest Editorial: next-generation analytical systems: devices and software.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Flow-programmable and reversible surface-induced LLPS in nanofluidic channels.

Lab on a chip·2026
Same author

Physics-guided machine learning for real-time, non-contact quantification of liquid volume at micro litter under cyclone flow.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Room temperature quantitative liquid concentration device and application to interleukins analysis in a B-cell culture medium.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2024
Same author

Room-temperature mL-to-μL quantitative liquid concentration device for cyclone flow.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2024
Same author

Nanofluidic Detection Platform for Simultaneous Light Absorption and Scattering Measurement of Individual Nanoparticles in Flow.

Analytical chemistry·2024

Related Experiment Video

Updated: Jan 30, 2026

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

12.3K

Femtoliter Gradient Elution System for Liquid Chromatography Utilizing Extended Nanofluidics.

Hisashi Shimizu1, Kouto Toyoda2, Kazuma Mawatari2

  • 1International Research Center for Neurointelligence , The University of Tokyo , 7-3-1, Hongo , Bunkyo, Tokyo 113-0033 , Japan.

Analytical Chemistry
|January 22, 2019
PubMed
Summary
This summary is machine-generated.

A new flexible gradient system enables femtoliter-scale protein separation using nanofluidic channels and HPLC pumps. This breakthrough advances high-efficiency chromatography for single-cell omics studies.

More Related Videos

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

5.0K
Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein
07:19

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Published on: April 26, 2024

3.8K

Related Experiment Videos

Last Updated: Jan 30, 2026

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

12.3K
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

5.0K
Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein
07:19

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Published on: April 26, 2024

3.8K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Nanotechnology

Background:

  • Miniaturization in chromatography enhances separation efficiency and instrument size.
  • Previous nanofluidic systems lacked flexible gradient design and protein separation capabilities.

Purpose of the Study:

  • To develop a flexible gradient system for femtoliter-scale protein separation.
  • To enable femtoliter-scale sample injection compatible with gradient elution.

Main Methods:

  • Utilized standard HPLC pumps and an auxiliary mixer for gradient generation.
  • Implemented a novel sample injection system for femtoliter-scale precision.
  • Applied the system to separate proteins from model and real samples.

Main Results:

  • Successfully demonstrated a flexible gradient system for nanofluidic chromatography.
  • Achieved femtoliter-scale sample injection compatible with gradient elution.
  • Validated protein separation from complex samples using the developed system.

Conclusions:

  • The developed system offers efficient, femtoliter-scale protein separation.
  • This technology is poised to significantly contribute to single-cell level omics studies.
  • The flexible gradient system overcomes previous limitations in nanofluidic chromatography.