Related Experiment Video
Updated: Jan 30, 2026

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
Published on: October 18, 2019
Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich
Xiaomin Xu1, Jiyun Chen, Bangrui Li
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn tanglijuang@hnu.edu.cn.
Abstract:
Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.
Related Concept Videos
Bioavailability Study Design: Absolute Versus Relative Bioavailability
Angular Momentum: Single Particle
Acid and Bases: Ka, pKa, and Relative Strengths
Subatomic Particles
Relative Strengths of Conjugate Acid-Base Pairs
Hybrid Zones

