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Double Immunohistochemistry and Digital Image Analysis

Pablo Moreno-Ruiz1, Lina Wik Leiss2,3, Artur Mezheyeuski4

  • 1Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. pablo.moreno.ruiz@ki.se.

Methods in Molecular Biology (Clifton, N.J.)
|January 23, 2019
PubMed

Immunohistochemistry (IHC) is a commonly used technique for protein detection in tissue sections. The method requires high-affinity antibodies that are specific for the target proteins of interest. More advanced IHC techniques have been developed to meet the need for simultaneous detection of more than one target protein in the same tissue section. This chapter provides general guidelines for double IHC staining of formalin-fixed, paraffin-embedded tissue sections. Chromogenic substrates are chosen based on their excellent contrast and compatibility with the subsequent digital image analysis.

Keywords:
AntibodyChromogenic substrateDigital image analysisDouble immunohistochemistryFFPE

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