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ADAR1p150 Forms a Complex with Dicer to Promote miRNA-222 Activity and Regulate PTEN Expression in CVB3-Induced Viral

Xincai Zhang1, Xiangting Gao2, Jun Hu3

  • 1Institute of Forensic Medicine, Soochow University, Suzhou 215021, China. xczhang1@stu.suda.edu.cn.

International Journal of Molecular Sciences
|January 24, 2019
PubMed

Insights

Adenosine deaminases acting on RNA 1 p150 (ADAR1p150) promotes microRNA-222 expression in viral myocarditis. This leads to reduced PTEN protein, revealing ADAR1p150

Area of Science:

  • Molecular Biology
  • RNA Metabolism
  • Cardiovascular Research

Background:

  • Adenosine deaminases acting on RNA (ADAR) enzymes regulate RNA metabolism.
  • ADAR1 is implicated in inflammation, cancer, and antiviral defense.
  • The specific role of ADAR1p150 in vascular disease, particularly viral myocarditis (VMC), is not well understood.

Purpose of the Study:

  • To investigate the expression and function of ADAR1p150 in viral myocarditis (VMC).
  • To elucidate the molecular mechanisms involving ADAR1p150 in the pathogenesis of VMC.

Main Methods:

  • Utilized a mouse model of viral myocarditis (VMC).
  • Examined ADAR1p150 expression in cardiomyocytes using techniques like coimmunoprecipitation.
  • Analyzed miRNA-222 and PTEN expression and their interactions using bioinformatics and cell culture experiments.

Main Results:

  • ADAR1p150 expression was significantly elevated in VMC cardiomyocytes.
  • ADAR1p150 was found to complex with Dicer and promote miRNA-222 expression in VMC.
  • miRNA-222 expression was increased in VMC, leading to decreased PTEN protein levels.
  • Bioinformatics and cell culture confirmed miRNA-222 targets PTEN, suppressing its expression.

Conclusions:

  • ADAR1p150 plays a critical role in VMC by complexing with Dicer and upregulating miRNA-222.
  • The ADAR1p150/miRNA-222 axis suppresses PTEN expression in VMC.
  • This study uncovers a novel function of ADAR1p150 in regulating gene expression during viral myocarditis.
Keywords:
ADAR1p150PTENmiRNA-222viral myocarditis

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