Related Experiment Video

Updated: Jan 30, 2026

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
09:02

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Published on: June 5, 2020

7.9K

Discussion on critical points for a tailored therapy to cure hepatitis C virus infection

Nadia Marascio1, Angela Quirino1, Giorgio Settimo Barreca1

  • 1Department of Health Sciences, Unit of Clinical Microbiology, Unit of Infectious and Tropical Diseases, Magna Graecia University, Catanzaro, Italy.

Clinical and Molecular Hepatology
|January 24, 2019
PubMed

Insights

Hepatitis C virus (HCV) treatment is improving, but some patients still experience failure due to resistance. This review examines effective pan-genotypic treatments, genotyping costs, and resistance-associated substitutions detection for HCV eradication.

Area of Science:

  • Hepatology
  • Virology
  • Infectious Diseases

Background:

  • Hepatitis C virus (HCV) remains a significant global health concern, affecting millions worldwide.
  • Direct-acting antiviral (DAA) therapies since 2011 have transformed HCV treatment, improving sustained virological response (SVR) rates and enabling eradication strategies.
  • Treatment failures, though infrequent, can occur due to resistance-associated substitutions (RASs), which may be pre-existing or emerge during therapy, potentially hindering eradication efforts.

Purpose of the Study:

  • To critically evaluate the effectiveness of pan-genotypic HCV treatments in real-world clinical settings.
  • To assess the cost-effectiveness of HCV genotyping for guiding treatment decisions.
  • To determine the utility of detecting RASs in viral quasispecies using next-generation sequencing (NGS) for optimizing HCV treatment outcomes.

Main Methods:

  • Review of recent scientific literature on HCV treatment, resistance, and diagnostics.
  • Analysis of clinical data regarding the efficacy of direct-acting antiviral therapies.
  • Discussion of next-generation sequencing approaches for identifying resistance-associated substitutions in Hepatitis C virus populations.

Main Results:

  • While DAA therapies offer high SVR rates, questions persist regarding the universal effectiveness of pan-genotypic regimens outside controlled trials.
  • The cost-effectiveness of routine HCV genotyping for RAS detection requires careful consideration in clinical practice.
  • NGS offers a powerful tool for detecting minority RAS populations, but its clinical utility and integration into treatment strategies are still evolving.

Conclusions:

  • Further research is needed to confirm the real-world effectiveness of pan-genotypic HCV therapies across diverse patient populations and clinical scenarios.
  • Optimizing HCV eradication strategies requires a balanced approach to treatment selection, diagnostic testing, and resistance surveillance.
  • The integration of advanced molecular diagnostics like NGS may play a crucial role in overcoming treatment challenges and achieving global HCV elimination goals.

Keywords:
Deep sequencingDirect-acting antiviralsGenetic variationResistance-associated substitutions

More Related Videos

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

12.1K
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

14.0K

Related Concept Videos

Curing of Concrete01:20

Curing of Concrete

The hydration of cement takes place within the water-filled capillary pores. However, environmental elements can disrupt this process by evaporating water from the concrete surfaces. Sealed concrete with a water-cement ratio below 0.5 experiences self-desiccation, leading to water loss. The water loss in concrete is mitigated by curing. This technique involves keeping the concrete saturated to maintain the necessary temperature and moisture conditions, to optimally fill the spaces in the cement...
369
Curing Methods01:26

Curing Methods

Concrete members with a small surface-to-volume ratio are cured by oiling and moistening the forms before casting the concrete member. These forms can be left in place for a prolonged period to prevent moisture loss, and can be wetted if made of a material suitable for wetting. If the forms are removed early, the concrete member is moistened and covered with polythene sheets to maintain moisture. For large horizontal concrete surfaces exposed to dry weather, a temporary covering is suspended...
296
Accelerated Curing of Concrete01:25

Accelerated Curing of Concrete

Accelerating concrete curing is achieved by applying heat and additional moisture. This process accelerates the hydration of the cement, resulting in an earlier strength gain in the concrete. Steam curing is a method wherein the concrete products are either transported through a chamber on a conveyor belt or encased in plastic, allowing steam at atmospheric pressure to circulate freely around them. This process begins with a phase of moist curing that typically lasts between 3 to 5 hours, after...
472
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.6K
What are Viruses?00:50

What are Viruses?

Overview
128.1K
Critical Region, Critical Values and Significance Level01:16

Critical Region, Critical Values and Significance Level

The critical region, critical value, and significance level are interdependent concepts crucial in hypothesis testing.
In hypothesis testing, a sample statistic is converted to a test statistic using z, t, or chi-square distribution. A critical region is an area under the curve in  probability distributions demarcated by the critical value. When the test statistic falls in this region, it suggests that the null hypothesis must be rejected. As this region contains all those values of the...
13.4K