Related Experiment Video

Updated: Jan 30, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.5K

Group A Streptococcus co-ordinates manganese import and iron efflux in response to hydrogen peroxide stress

Andrew G Turner1, Karrera Y Djoko1,2, Cheryl-Lynn Y Ong1,3

  • 1School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Australia.

The Biochemical Journal
|January 24, 2019
PubMed

Insights

Group A Streptococcus uses manganese import (MtsABC) and iron export (PmtA) to ensure its superoxide dismutase (SodA) is correctly activated with manganese, crucial for combating oxidative stress during infection.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Oxidative Stress Response

Background:

  • Bacterial pathogens face challenges like metal imbalance and oxidative stress during infection.
  • Group A Streptococcus (GAS) must manage intracellular metal levels for survival and virulence.

Purpose of the Study:

  • To investigate how GAS regulates manganese and iron homeostasis under oxidative stress.
  • To elucidate the role of specific transporters and regulators in maintaining superoxide dismutase function.

Main Methods:

  • Analysis of MtsABC manganese import system regulated by MtsR.
  • Investigation of the PerR-regulated iron efflux system PmtA.
  • Assessment of SodA activity under varying metal conditions and stress.

Main Results:

  • GAS utilizes MtsABC for Mn(II) import under hydrogen peroxide stress.
  • SodA requires manganese for optimal activity; iron incorporation reduces its function.
  • PmtA is essential for reducing intracellular iron and preventing SodA "mismetallation" under high iron conditions.

Conclusions:

  • MtsR-regulated Mn(II) import and PerR-regulated Fe(II) efflux work coordinately.
  • This coordinated action ensures proper Mn(II) metallation of SodA for effective superoxide dismutase function in GAS.
Keywords:
Streptococcus pyogenesironmanganeseoxidative stresssuperoxide dismutases

More Related Videos

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells06:39

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells

Published on: October 20, 2023

3.9K
Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes08:45

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

Published on: May 10, 2022

2.4K

Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.6K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
Stress Response System01:21

Stress Response System

The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's...
879
Psychological Responses to Stress01:20

Psychological Responses to Stress

Psychological responses to stress encompass the various cognitive and emotional reactions individuals experience when faced with challenging or threatening situations, such as a job loss. Prolonged exposure to stressors can disturb emotional balance, increasing negative emotions (e.g., anxiety and sadness) and diminishing positive emotions (e.g., joy and satisfaction). These persistent emotional shifts are associated with an increased risk of both physical illness and mental health issues, such...
692
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
395
Ordinal Level of Measurement00:55

Ordinal Level of Measurement

The way a set of data is measured is called its level of measurement. Correct statistical procedures depend on a researcher being familiar with levels of measurement. For analysis, data are classified into four levels of measurement—nominal, ordinal, interval, and ratio.
Data measured using an ordinal scale are similar to nominal scale data, but there is one major difference. The ordinal scale data can be ordered. An example of ordinal scale data is a list of the top five national parks...
33.8K